重组人PD-1蛋白(Human PD-1 Protein)

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators.

100ug/¥2680 500ug/询价
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产品货号:TL-732

重组人PD-1蛋白(Recombinant Human PD-1 Protein),GMP级

 

产品描述(Description)

 

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer。

 

 

分子别名(Synonym)

 

PDCD1、CD279、SLEB2

 

 

纯度(Purity)

 

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

 

 

内毒素(Endotoxin)

 

≤0.01EU/ug

 

  

表达宿主(Expression host)

  

人HEK293细胞

 

 

来源(Source)

 

Human

 

 

分子量(Molecular Weight)

 

Human PD-1 Protein 包含380个氨基酸,预测理论分子量为43.4kd

 

 

蛋白序列(Protein Sequence)

  

DNA序列编码人PD-1(NCBI Reference Sequence: NP_005009.2)表达带有Fc标签在C末端

 

 

生物活性(Biological Activity)

 

固定人PD-1(Fc标签)0.5μg/mL(100μL/孔)能够结合人PD-L1(His标签)的线性范围是0.5-10μg/mL

 

 

稳定性(Stability)

 

冻干的样本的可以在4℃保存24个月,溶解后的液体可以于-20℃保存6-12个月,并且避免反复冻融

 

 

组成(Component)

 

含有6%甘露醇的无菌PBS(PH7.4)的冻干粉

 

 

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