rmouse CD14(rmouse CD14)

10ug
咨询电话 010-88681766

产品货号:012-1

产品描述   Characteristic

 

The mCD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

 

产品描述   mCD14-content

 

1 mg/ml phosphate-buffered saline , pH 7.2, (determined by mouse-CD14-ELISA lyophilized

For reconstitution add 10µl water to obtain 1 mg/ml

 

产品描述   Purification

 

Purified by His-tag with metal affinity chromatography Talon (Clontech) and controlled by SDS page

 

产品描述   Endotoxin content

 

Below 0.1 ng/ml after heating 5', 75°C (LAL-test, Chromogenix)

 

产品描述   Special note

 

The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein.
Up to 5µg/ml mouseCD14 inhibit binding of FITC-LPS (0.5µg/ml) to mouse CD14+CHO transfectants

 

产品描述   Quantity

 

10 µg

 

产品描述   Storage

 

long time storage at –80°C

 

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