The mCD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.
1 mg/ml phosphate-buffered saline , pH 7.2, (determined by mouse-CD14-ELISA lyophilized
For reconstitution add 10µl water to obtain 1 mg/ml
Purified by His-tag with metal affinity chromatography Talon (Clontech) and controlled by SDS page
Below 0.1 ng/ml after heating 5', 75°C (LAL-test, Chromogenix)
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein.
Up to 5µg/ml mouseCD14 inhibit binding of FITC-LPS (0.5µg/ml) to mouse CD14+CHO transfectants
long time storage at –80°C
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unpurified control to 011-3a
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